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templiphi 100 amplification kit  (Cytiva Europe)


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    Structured Review

    Cytiva Europe templiphi 100 amplification kit
    Templiphi 100 Amplification Kit, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 94/100, based on 271 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/templiphi+amplification+kit/TempliPhi+100+amplification+kit/custom%4025640010%4042486980
    Average 94 stars, based on 271 article reviews
    templiphi 100 amplification kit - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Selection:

    Article Title: Efficient screening of anti-idiotype DNA aptamers that bind specifically to trastuzumab for bioanalytical applications
    Article Snippet: The ligation products were added to Jet Competent E. coli cells (DH5α, BioDynamics Laboratory Inc. Tokyo, Japan) and transformed. .. White colonies with inserted inserts were collected by color selection and sequenced by contract analysis (Eurofins Genomics, Louisville, KY, USA) after preparing samples for sequencing using the TempliPhi Amplification Kit (GE Healthcare, Chicago, IL, USA). ..

    Sequencing:

    Article Title: Efficient screening of anti-idiotype DNA aptamers that bind specifically to trastuzumab for bioanalytical applications
    Article Snippet: The ligation products were added to Jet Competent E. coli cells (DH5α, BioDynamics Laboratory Inc. Tokyo, Japan) and transformed. .. White colonies with inserted inserts were collected by color selection and sequenced by contract analysis (Eurofins Genomics, Louisville, KY, USA) after preparing samples for sequencing using the TempliPhi Amplification Kit (GE Healthcare, Chicago, IL, USA). ..

    Article Title: Genomic SSR marker development in lentil (Lens culinaris Medik.) and assessment of cross-species/genera transferability to related legumes
    Article Snippet: .. Positive plasmids were amplified using the TempliPhi Amplification kit (GE Healthcare, Wauwatosa, USA) and sequenced using the BigDye Terminator v3.1 Cycle Sequencing Kit in an Applied Biosystems Prism 3500 Genetic Analyzer System (Applied Biosystems, Foster City, USA). .. Vector sequences were removed using Vecscreen (https://www.ncbi.nlm.nih.

    Amplification:

    Article Title: Efficient screening of anti-idiotype DNA aptamers that bind specifically to trastuzumab for bioanalytical applications
    Article Snippet: The ligation products were added to Jet Competent E. coli cells (DH5α, BioDynamics Laboratory Inc. Tokyo, Japan) and transformed. .. White colonies with inserted inserts were collected by color selection and sequenced by contract analysis (Eurofins Genomics, Louisville, KY, USA) after preparing samples for sequencing using the TempliPhi Amplification Kit (GE Healthcare, Chicago, IL, USA). ..

    Article Title: Coding complete genomes of an iridovirus and two parvoviruses identified in lab-reared social spiders ( Stegodyphus dumicola )
    Article Snippet: Viral DNA was extracted from 200 μL of the filtrate using the Roche high pure viral nucleic acid kit (Roche, USA) following the manufacturer’s instructions. .. The viral DNA was amplified using rolling circle amplification (RCA) with the TempliPhi amplification kit (GE Healthcare, USA). .. The RCA products were combined with the viral nucleic acid at a 1:1 ratio, and ~10 ng of DNA was used to prepare Illumina sequencing libraries using the Nextera DNA flex kit (Illumina, USA) and were sequenced on an Illumina NovaSeq 6000 (2 × 150 bp paired-end library), yielding 14,922,720 paired-end reads.

    Article Title: Somatic mobility of transposons is explosive and shaped by distinct integration biases in Arabidopsis thaliana
    Article Snippet: .. The air dried eccDNA pellet was resuspended in TempliPhi Sample buffer and rolling circle amplification was performed using TempliPhi Amplification kit (Cytiva) by incubation at 28 °C for 65 h. The reaction was terminated by inactivating enzyme at 70 °C for 30 min. ..

    Article Title: Structure-guided mutagenesis of the capsid protein indicates that a nanovirus requires assembled viral particles for systemic infection
    Article Snippet: .. 1 μL of this solution was then used as template for rolling circle amplification (RCA) using the TempliPHi Amplification Kit (GE Healthcare) according to the manufacturer instructions. ..

    Article Title: Successful large gene augmentation of USH2A with non-viral episomal vectors.
    Article Snippet: .. Vector isolation and episomal analysis For detection of episomally maintained DNA vectors, the TempliPhi Amplification Kit (GE Healthcare) was used, following the manufacturer’s protocol. .. Total DNA was extracted with the Wizard Genomic DNA Purification Kit (Promega) and quantified using a NanoDrop 2000c (Thermo Fisher Scientific) spectrophotometer.

    Article Title: Molecular typing of bovine papillomaviruses in Costa Rica.
    Article Snippet: Bovine papillomaviruses are related to cause fibroepithelial proliferations in the skin and mucosae and are associated with economic loss mainly related to poor body condition and reduced milk production.. This study aimed to investigate the presence and types of bovine papillomaviruses (BPVs) in cattle sampled in different areas of Costa Rica using molecular techniques.. A descriptive study with a non-probability convenience sampling was carried out.

    Article Title: Genomic SSR marker development in lentil (Lens culinaris Medik.) and assessment of cross-species/genera transferability to related legumes
    Article Snippet: .. Positive plasmids were amplified using the TempliPhi Amplification kit (GE Healthcare, Wauwatosa, USA) and sequenced using the BigDye Terminator v3.1 Cycle Sequencing Kit in an Applied Biosystems Prism 3500 Genetic Analyzer System (Applied Biosystems, Foster City, USA). .. Vector sequences were removed using Vecscreen (https://www.ncbi.nlm.nih.

    Incubation:

    Article Title: Somatic mobility of transposons is explosive and shaped by distinct integration biases in Arabidopsis thaliana
    Article Snippet: .. The air dried eccDNA pellet was resuspended in TempliPhi Sample buffer and rolling circle amplification was performed using TempliPhi Amplification kit (Cytiva) by incubation at 28 °C for 65 h. The reaction was terminated by inactivating enzyme at 70 °C for 30 min. ..

    Isolation:

    Article Title: Successful large gene augmentation of USH2A with non-viral episomal vectors.
    Article Snippet: .. Vector isolation and episomal analysis For detection of episomally maintained DNA vectors, the TempliPhi Amplification Kit (GE Healthcare) was used, following the manufacturer’s protocol. .. Total DNA was extracted with the Wizard Genomic DNA Purification Kit (Promega) and quantified using a NanoDrop 2000c (Thermo Fisher Scientific) spectrophotometer.



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